Figure 2
Expression of neuropeptides in LepRLH cells and co-localization patterns in the LH. (A) Fixed coronal sections from LepR-Cre x L-tdTomato mice underwent fluorescent in situ hybridization (FISH) with anti-sense RNA probes for Nts, Gal, Cartpt, Tac1 and Crh ("Neuropeptide"—red channel) followed by immunohistochemistry (IHC) for tdTomato ("LepR"—green channel) and imaging. The percentage of Neuropeptide+LepR+ cells out of the total LepR+ in the LH was quantified, as represented in the barplots (mean, n = 3 hypothalami). (B) Localization of Neuropeptide+LepR+ cells was observed under the microscope and drawn on representative graphic images of coronal sections from the mouse Brain Atlas58, which were adapted for this figure. Arc: Arcuate nucleus of the Hypothalamus, 3 V: 3rd Ventricle, DMH: Dorsomedial Hypothalamus, VMH: Ventromedial Hypothalamus, LH: Lateral Hypothalamus, PeF: Perifornical area, f: Fornix, MTu: Medial Tuberal nucleus.
