Figure 5 | Scientific Reports

Figure 5

From: A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy

Figure 5

Specificity and sensitivity of the PCR-LFA for the detection of PCR amplicons generated with a set of primers, A397F, C357F and R502. (A) Image of PCR amplicons as detected by 1.7% agarose gel electrophoresis. 1: A. pierrei, 2: A. pothieri, 3: A. acuminata, 4: A. gigantea. 5: A. grandiflora, 6: A. cambodiana, 7: A. littoralis, 8: A. ringens, 9: A. tentaculata, 10: C. pulchellum, 11: T. scabra, 12: J. sambac, 13: J. adenophyllum, 14: no-DNA template as a control reaction. (B) T2 line was detected from Aristolochia spp. samples (1–9) but not detected from non-Aristolochia samples (10–14). (C) Sensitivity of the Aristolochia PCR-LFA. Template DNA: 2, 1, 0.1 and 0.01 ng. NTC: no-DNA template.

Back to article page