Figure 2
From: R-spondin3 is a myokine that differentiates myoblasts to type I fibres
Rspo3 treatment increases MyHC I expression in myotubes. (A) Experimental scheme. Mouse primary myoblasts derived from satellite cells of type I or type II fibres were cultured for 3 d in a differentiation media containing BSA (200 ng/mL; Control) or mouse Rspo3 recombinant protein (200 ng/mL; 6.45 nM). (B) Protein levels of MyHC I and MyHC II in the differentiated myotubes were measured by Western blotting. The expression of these proteins was normalised to that of β-actin. Values are presented as mean ± SEM (n = 6 mice for analysing MyHC I, n = 4–5 mice for analysing MyHC II). **P < 0.01 by Student’s t-test. Uncropped blots can be found in Supplementary Fig. 9.
