Figure 3
From: R-spondin3 is a myokine that differentiates myoblasts to type I fibres
Rspo3 activates the Wnt/β-catenin signalling pathway during myogenic differentiation. (A, B) Mouse primary myoblasts derived from satellite cells of type I and type II fibres were differentiated for 3 d in the absence (BSA; 200 ng/mL) or presence of the mouse Rspo3 recombinant protein (200 ng/mL; 6.45 nM). Cytoplasmic and nucleic fractions were extracted from myotube lysate. Protein levels of β-catenin, lamin A/C (nuclear marker), and α-tubulin (cytosol marker) in myotubes were analysed using Western blotting. Values are presented as mean ± SEM (n = 10–12 mice for analysing cells derived from type I fibres, n = 8 mice for analysing cells derived from type II fibres). *P < 0.05; **P < 0.01 by Student’s t-test. Uncropped blots can be found in Supplementary Fig. 10.
