Figure 4

Rspo3 does not activate non-canonical Wnt signalling pathways during myogenic differentiation. (A) Mouse primary myoblasts derived from satellite cells of type I and type II fibres were differentiated for 3 d in the absence (BSA; 200 ng/mL) or presence of the mouse Rspo3 recombinant protein (200 ng/mL; 6.45 nM). Protein levels of phospho-JNK1/2/3 and pan-JNK1/2/3 in myotubes were analysed using Western blotting. Values are presented as mean ± SEM (n = 6 mice). (B) Mouse primary myoblasts derived from satellite cells of type I and type II fibres were cultured for 3 d in the differentiation media as described in (A). Protein levels of NFATc1 in myotubes were analysed using Western blotting. Values are presented as mean ± SEM (n = 12 mice for analysing cells derived from type I fibres, n = 10 mice for analysing cells derived from type II fibres). Uncropped blots can be found in Supplementary Fig. 11.