Figure 4 | Scientific Reports

Figure 4

From: Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells

Figure 4

The anti-PD-1 29F.1A12 and RMP1-30 antibody clones recognize PD-1 surface protein on live YUMM melanoma cells. (a) Relative PD-1 gene (Pdcd1) expression (mean ± s.d., two distinct primer sets) in wild-type B16-F10, YUMMER1.7D4, YUMM1.7, YUMM1.G1, YUMM3.3, YUMM4.1, and YUMM5.2 cells. (b,c) Representative FACS plots (YUMM3.3) of surface PD-1 protein expression (clones 29F.1A12 and RMP1-30, respectively) or isotype control staining and % positivity (mean ± s.e.m.) on live versus dead (fixable viability dye, FVD-negative or -positive, respectively) YUMM melanoma cell lines, as in (a).

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