Figure 1 | Scientific Reports

Figure 1

From: Association of circulating gene expression signatures with stiffness following total knee arthroplasty for osteoarthritis: a pilot study

Figure 1

Study workflow. (A) We collected peripheral blood mononuclear cells (PBMCs) and PAXgene blood RNA tubes the day of surgery (DOS) and at 24 h after surgery (POD1) in patients with knee osteoarthritis (OA) undergoing total knee arthroplasty (TKA). At 6 weeks after surgery, patients were classified as cases and controls based on range-of-motion (ROM). Stiffness was defined as ROM ≤ 95° measured by goniometer. (B) RNA isolated from PBMCs was used for NanoString gene expression analyses to compare POD1 and DOS gene expression profiles. (C) RNA isolated from PAXgene blood RNA tubes was used for RNA-seq, comparing POD1 vs. DOS samples from controls and cases. Created with BioRender.com.

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