Figure 5 | Scientific Reports

Figure 5

From: Association of circulating gene expression signatures with stiffness following total knee arthroplasty for osteoarthritis: a pilot study

Figure 5

Functional analyses of the differentially expressed genes identified by RNA-seq in PAXgene blood RNA in cases (stiff knees) and controls. (A) Representation of the QuSAGE pathway analyses at 24 h after surgery (POD1) relative to the day of surgery (DOS) in control patients (N = 10) showing functional pathways differentially expressed at POD1 vs. DOS (FDR < 0.01). Representative genes associated with the (B) ATF2, (C) INFLAM, (D) canonical NF-κB signaling, and (E) activated TLR4 signaling pathways are shown, representing changes in gene expression (logFC) in POD1 vs. DOS for the controls. (F) Transcription factor regulatory network analysis using differentially expressed genes (logFC > 1, FDR < 0.05) at POD1 compared to DOS in the control group. (G) Representation of the QuSAGE pathway analyses in POD1 relative to DOS in cases (N = 8). Pathways that displayed differential expression (FDR < 0.01) only in the cases are highlighted. Representative genes associated with the (H) matrisome associated and (I) steroid hormone biosynthesis pathways are shown, representing changes in gene expression (logFC) in POD1 vs. DOS for the cases. Graphs were created with GraphPad Prism 8 (GraphPad Software, San Diego, CA).

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