Figure 2

(a) A scatter plot of size on the horizontal axis and refractive index on vertical axis for a yeast sample before the addition of alcohol. Each point on the plot represents a single particle that flowed through the viewing region of the microfluidic chip during THC analysis. The colored boxed are user-defined regions of interest. The points colored in orange represent dead yeast cells and the points colored in cyan represent live yeast cells. Particles outside of the user-defined boxes are colored gray. (b) Density distributions of particle size for the sample shown in (a). The orange, cyan and gray curves represents the size density distributions of dead cells, live cells, and all particles respectively. The area under each curve for a given size range represents the number of particles (of the species represented by that curve) in that size range. The peak of each curve shows the most common size of each particle type. (c) Density distributions of particle refractive index for the sample shown in (a). The coloring is the same as in (b). The area under each curve for a given refractive index range represents the number of particles (of the species represented by that curve) in that refractive index range. The peak of each curve shows the most common refractive index of each particle type. (d)–(f) Scatter plot, size density plot, and refractive index density plot as in (a)–(c) but for a yeast sample that was exposed to 15% isopropanol by volume for 71 min.