Figure 1 | Scientific Reports

Figure 1

From: Biosensing firefly luciferin synthesis in bacteria reveals a cysteine-dependent quinone detoxification route in Coleoptera

Figure 1

(Panels 0 to ~ 90 min) CCD bioluminescence detection of in vitro luciferin formation from d-cystein and p-benzoquinone (wells 3). (Panel “FLUORESCENCE”) fluorescence of luciferin upon UV irradiation also indicates luciferin formation from d-cysteine and p-benzoquinone in well 3, and from l-cysteine and p-benzoquinone in well 5. (1) Buffer + MgATP; (2) Buffer + MgATP + AmyLuc; (3) Buffer + MgATP + AmyLuc + d-cys + BQ; (4) Buffer + MgATP + AmyLuc + d-Cys; (5) Buffer + MgATP + AmyLuc + l-cys + BQ; (6) Buffer + MgATP + AmyLuc + l-cys; (7) Buffer + MgATP + AmyLuc + l-cys; (8) Buffer + MgATP + AmyLuc + d-cys + Hydroquinone; (9) Buffer + MgATP + AmyLuc + l-cys + Hydroquinone; (10) Buffer + MgATP + AmyLuc + Hydroquinone.

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