Figure 4
From: Development of a novel light-up probe for detection of G-quadruplexes in stress granules
Cellular images of fixed H1299 cells. Co-staining for 1 and BG4: (A) 1, (B) BG4, (C) magnified image for BG4, and (D) merged image. Co-staining for anti-fibrillarin antibody, BG4, and 4ʹ,6-diamidino-2-phenylindole (DAPI): (E) anti-fibrillarin antibody, (F) BG4, (G) DAPI, and (H) merged image. Fluorescence was analyzed using the following detection channels: “compound 1 channel” (ex. = 360/40 nm, em. = 605/70 nm), “BG4 channel” (ex. = 470/40 nm, em. = 525/50 nm), “Fibrillarin channel” (ex. = 560/40 nm, em. = 630/75 nm), and “DAPI channel” (ex. = 360/40 nm, em. = 460/50 nm).
