Figure 5

Authentication of the 982 mtDNA fragments assigned to Cervidae in sample SP5773 from Layer V. Following the alignment of the fragments to the Cervus elaphus reference mitochondrial genome and their filtering for a mapping quality of 25 or more, 965 fragments were retained for these analyses. (a) Frequency of nucleotide substitutions towards the starts and ends of fragments compared to the reference genome. (b) Base composition towards the start and end of the sequenced DNA fragments (within dashed gray outline) and of the reference genome upstream and downstream from fragments’ ends. (c) Length distribution (in bp) of the Cervidae mtDNA fragments. The average length is shown by the red dashed line.