Table 2 Influence of different nitrogen sources on dry biomass, GLA and lipid production parameters by coculture fermentation.
Parameter | Organic nitrogen sources | In-organic nitrogen sources | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Ammonium tartrate | Yeast extract | Malt extract | Peptone | Casein | Urea | Sodium nitrate | Ammonium chloride | Ammonium sulfate | Axenic fungus (control)* | |
Dry biomass (g/L) | 10.5 ± 0.2 | 9.14 ± 0.1 | 9.12 ± 0.1 | 10.88 ± 0.2 | 9.66 ± 0.1 | 10.24 ± 0.1 | 8.93 ± 0.1 | 10.88 ± 0.2 | 12.95 ± 0.11 | 10.4 ± 0.22 |
Lipid yield (g/L) | 2.83 ± 0.01 | 1.91 ± 0.01 | 2.69 ± 0.01 | 2.72 ± 0.01 | 2.51 ± 0.02 | 1.48 ± 0.01 | 2.50 ± 0.02 | 2.44 ± 0.01 | 3.62 ± 0.03 | 2.34 ± 0.04 |
Specific yield of lipid (Yp/x) | 0.27 ± 0.0 | 0.21 ± 0.01 | 0.29 ± 0.0 | 0.25 ± 0.01 | 0.26 ± 0.02 | 0.14 ± 0.0 | 0.28 ± 0.02 | 0.22 ± 0.0 | 0.28 ± 0.02 | 0.22 ± 0.03 |
Lipid content (%) | 27 ± 0.35 | 21 ± 0.18 | 29.5 ± 0.50 | 25 ± 0.22 | 26 ± 0.38 | 14.5 ± 0.10 | 28 ± 0.25 | 22.5 ± 0.35 | 28 ± 0.33 | 22.5 ± 1.30 |
GLA content (%, TFA) | 13.7 ± 0.1 | 11.54 ± 0.3 | 11.53 ± 0.2 | 12.43 ± 0.3 | 11.89 ± 0.2 | 16.23 ± 0.2 | 11.15 ± 0.1 | 10.71 ± 0.1 | 13.39 ± 0.1 | 9.5 ± 0.1 |
Volumetric biomass production rate,Qx (g/L/d) | 2.62 ± 0.01 | 2.28 ± 0.01 | 2.28 ± 0.01 | 2.72 ± 0.02 | 2.41 ± 0.01 | 2.56 ± 0.02 | 2.23 ± 0.02 | 2.72 ± 0.02 | 2.23 ± 0.01 | 2.6 ± 0.05 |
Volumetric lipid production rate (Qp) | 0.70 ± 0.01 | 0.47 ± 0.0 | 0.67 ± 0.01 | 0.68 ± 0.02 | 0.62 ± 0.01 | 0.37 ± 0.0 | 0.62 ± 0.01 | 0.61 ± 0.01 | 0.90 ± 0.03 | 0.59 ± 0.02 |
