Figure 2
From: Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system
(A) Fluorescence traces of Cas13a activation assay for each crRNA from the 0056 set on a single multi-well plate. Solid lines represent mean and shaded regions standard deviation (n = 3). Each crRNA was tested with a control run lacking target RNA refered to as “Control (no target)” and an experimental run refered to as “Experimental (with target)”. Each plate contains an internal negative control lacking crRNA labeled “Control ( −)” and an internal positive control with crRNA 0056–07 labeled “Control ( +)”. (B) Summary plot of flourescence throughout the experiment, where the difference in sums per sample between “Control (no target)” and “Experimental (with target)” are normalized against the difference of the internal controls. Bars represent mean values and standard deviation is represented by error bars (n = 3). p-values < 0.05 determined by analysis of variance and Tukey test against internal control are denoted by *.
