Figure 4
From: Functional human cell-based vascularised cardiac tissue model for biomedical research and testing
Cardiomyocyte gene expression profile reached a more mature state in cardiac tissue model than in monocultured cardiomyocytes. (a) PCA plot of cardiomyocyte monoculture (CM day 1 & CM day 7) and cardiac tissue model (CTM day 1 & CTM day 7) samples using top 500 most variable genes and variance stabilizing transformation (DESeq2). Numbers attached to the principal components correspond to the percentage of variance that is attributed to it. (b) Heatmap of the log2-transformed fold changes (day 1 to day 7) of the cardiomyocyte monoculture and cardiac tissue model samples. Pre-selected genes of interest are annotated according to category and typical site of expression. Values for genes with a statistically insignificant fold change (padj > 0.05) are not reported. (c) Heatmap of expression changes per gene for each sample. The DESeq2-normalized count of each gene was additionally normalized against the mean of the day 1 expression of each gene. Day 1 normalization was done separately for CM and CTM, followed by a log2 transformation. Genes and their annotations correspond to those of (b). Number of replicates from two repeats n = 4 for CTM day 1, CM day 1 and CM day 7; n = 3 for CTM day 7. The heatmaps were created in R 3.6.0 using pheatmap v1.0.12 (https://github.com/raivokolde/pheatmap).
