Table 1 List of the used antibodies.
From: Analysis of genomic alterations in cancer associated human pancreatic stellate cells
Primary antibodies | Origin | Cone | Company |
|---|---|---|---|
α – smooth muscle actin | Mouse-monoclonal antibody Clon 1A4 | 8 μg/ml | Dako Denmark |
Periostin | Rabbit-polyclonal antibody | 2 μg/ml | Biovendor Germany |
SERPINB2 | Rabbit-polyclonal antibody | 0,39 mg/ml | Thermo Fisher Scientific, USA |
GFAP | Rabbit-polyclonal antibody | 1:1000 (IF) | Abcam UK |
Second antibody LSAB-Kit | Biotinylated anti-rabbit and anti-mouse immunoglobulin | Dako Germany | |
Alexa Fluor488 | Goat anti Rabbit IgG H&L | 2 μg/mL stock | Abcam UK |
Hoechst | Nucleus coulor 33,342 | 10 mg/ml stock | Sigma-Aldrich Germany |
