Figure 2
Cryopreservation experiments with Mytilus galloprovincialis 72 h-old D-larvae to test the effect of different CPA combinations. (A) Relative percentage of abnormal D-larvae compared to the control group developed 48 h from 72 h-old D-larvae exposed for 60 min to different cryoprotecting combinations of Ethylene–Glycol (EG), trehalose (TRE) and Polyvinylpyrrolidone (PVP) in Filtered Sea Water (FSW) or Milli-Q Water in 72 h-old D-larvae (% of abnormal larvae in controls: 15 ± 9.29). (B) Percentage of 72 h-old D-larvae developing to normal D-larvae following cryopreservation. Cryopreservation success was assessed as the average percentage of normal D-larvae after 48 h post cryopreservation in comparison to control group (Mean ± Standard Deviation (SD), n = 100, 3 replicates per treatment). Asterisks or letters show significant differences between control group and treatments. Different letters or asterisks show significant differences with p < 0.05 and in all cases lower % of normal larvae than controls (93 ± 6.33%).
