Figure 3

Relative percentage of M. galloprovincialis normal D-larvae compared to control group developed from cryopreserved trochophores (A) and 72 h-old D-larvae (B) using different cooling rates after the seeding (a.s.) check (− 1 °C/min, − 0.5 °C/min and − 0.3 °C/min) tested in combination with increasing thawing baths at 18 °C, 28 °C, 35 °C and 40 °C for melting the ice. Results are expressed as Mean ± Standard Deviation (SD), n = 100, 3 replicates per treatment. Letters show significant differences between treatments (p < 0.05).