Figure 7
Fitness parameters (survival, larval shell size and settlement success) assessed over the long-term experiment carried out with F2 Mytilus galloprovincialis 72 h-old D-larvae. (A) Relative percentage of normal D-larvae obtained at day 2 of incubation after F2 72 h-old larval cryopreservation compared with control group. Mean ± Standard Deviation, SD (n = 100, 3 replicates per treatment). (B) Box plots for the distribution of shell sizes in microns (µm) of post-thawed larvae. The line inside the box represents the median value and box ends correspond to upper and lower quartiles. Upper and lower whiskers represent values outside the middle 50% quantile (n = 35, 3 replicates per treatment). (Aʹ,Bʹ,Aʹʹ,Bʹʹ) Post-thaw parameters of Mytilus galloprovincialis larvae after 72 h-old D-larval cryopreservation during 20 days of larval rearing. (Aʹ) Survival percentages (Mean ± Standard Deviation (SD), n = 3 tanks per treatment (150 L)). (Bʹ) Shell lengths (Mean ± Standard Deviation (SD), n = 3 tanks per treatment (150 L)) of developed larvae for 20 days. (Aʹʹ) Percentages of settled larvae per treatment (Mean ± Standard Deviation (SD), n = 3 tanks per treatment (50 cm3), initially, 1284 larvae per replicate). Percentage of settlement expressed as the proportion of juveniles obtained from the sub-sample of competent larvae studied in each treatment. (Bʹʹ) Box plots for the distribution of shell sizes in microns (µm) of settled juveniles. The line inside the box represents the median value and box ends correspond to upper and lower quartiles. Upper and lower whiskers represent values outside the middle 50% quantile (n = 35, 3 settlement drums per treatment (50 cm3)). F1CRYO-F2CT control larvae obtained from adults developed from cryopreserved larvae, F1CRYO-F2CRYO cryopreserved larvae obtained from adults developed from cryopreserved larvae, F1CT-F2CT control larvae obtained from adults developed from control larvae, F1CT-F2CRYO cryopreserved larvae obtained from adults developed from control larvae. Statistical significance was considered when p < 0.05.
