Figure 1

The comparative HPLC chromatograms of Dolutegravir developed using a mixture of water, acetonitrile and methanol in the ratio of 20:40:40 with 0.2% formic acid as the mobile phase. (A) System suitability; (B) Dolutegravir with internal standard; (C) low-level; (D) mid-level; (E) high-level of DTG concentrations.