Figure 1

Unique 4D Dataset of lymphocytes inside fresh human lymphoid tissue sections. (E) represents the raw data (totaling 24 video sequences where only the first frame is shown). Each sample (i.e. a single video sequence) consists of three channels (A–C). Channel B shows CD35 positive follicular dendritic cells, which were used for context analysis. Channel A and C describe a single stain among T-cells (CD3), B-cells (CD20), and follicular T-helper cells (PD1). Moreover, from these channels, cell tracking data is extracted. Specifically, the cell tracks shown in (D) and (F) are computed from T- and B-cells, respectively. More details are described in “Material and methods”.