Figure 5
Functional information hidden in the context of cell movement. The rows A and B show the full evaluation of two videos, including traditional 2D morphology and the new movement characteristics. (A.1) shows a dense region near follicular dendritic cells filled with B cells and PD1 positive cells. (B.1) represents a sparse region mainly filled with T cells. The morphologies of the present cells are round and compact. Inspecting tracks and DD clusters of B and T cells inside both regions (A.2 and B.2), we can see an over representation of long and directed tracks inside (B.2). compared to (A.2). The over representation of directed DD clusters indicate a more active region. These hints can be confirmed by the collective behaviour of B cells, T cells and PD1 positive cells. We visualized the optical flow of the last 30 frames of Video A (A.3) and B (B.3). A.3 reveals a localized circular movement pattern indicating patrolling cells inside an interfollicular region, schematically show in figure (A.4). (B.3) reveals an in and outflux of T cells and B cells from a lymphoid follicle, schematically shown in figure (B.4). Here, we can show, that DD clusters and collective movement patterns has the possibility to reveal cellular states and functions, which can not be detected by the evaluation of cell morphologies inside static images.
