Table 2 Used antibodies in immunofluorescence (IF) and Western blotting (WB).
Target | Company | Catalog number | Application | Concentration |
|---|---|---|---|---|
Mouse anti-human collagen type I | Abcam, Cambridge, United Kingdom | ab90395 | IF | 1:1000 |
Mouse anti-human α-smooth muscle actin (α-SMA) | Dako, Glostrup, Denmark | M0851 | IF, WB | 1:500 |
Mouse anti-human fn-EDA | Abcam, Cambridge, UK | ab6328 | IF | 1:1000 |
Goat anti-human collagen type I | Santa Cruz Biotechnology, Dallas, Texas, USA | sc-8783 | WB | 1:1000 |
Mouse anti-human YAP | Santa Cruz Biotechnology, Dallas, Texas, USA | sc-101199 | WB | 1:500 |
Mouse anti-human Smad2 | Cell Signaling Technology, Leiden, The Netherlands | 3103S | WB | 1:1000 |
Rabbit anti-human Smad3 | Abcam, Cambridge, UK | Ab28379 | WB | 1:1000 |
Rabbit Anti- human YWHAZ | Abcam, Cambridge, UK | ab51129 | WB | 1:1000 |
Biotinylated, goat Anti-mouse IgG | SouthernBiotech, Birmingham, AL, USA | 1031–08 | IF | 1:500 |
Streptavidin-CY3 | SouthernBiotech, Birmingham, AL, USA | 7100–12 | IF | 1:1000 |
Polyclonal Goat anti-rabbit Immunoglobulins/HRP | Dako, Glostrup, Denmark | P0448 | WB | 1:5000 |
Polyclonal Rabbit anti-goat Immunoglobulins/HRP | Dako, Glostrup, Denmark | P0449 | WB | 1:5000 |
Polyclonal Goat anti-mouse Immunoglobulins/HRP | Dako, Glostrup, Denmark | P0447 | WB | 1:5000 |
