Figure 2 | Scientific Reports

Figure 2

From: Development of actionable targets of multi-kinase inhibitors (AToMI) screening platform to dissect kinase targets of staurosporines in glioblastoma cells

Figure 2

STS derivatives screening in Chaperone interaction assay. (A) Representative images of colony formation assay in T98G cells treated vehicle (DMSO) and NZ-8-061 in combination with STS derivatives. (B) A scheme of Chaperone interaction assay. Kinases tagged with 3xFLAG (blue), Renilla-CDC37 (red), tested drugs (green), anti-FLAG antibodies (grey). (C) Heat map representation of interaction of STS derivatives, CEP-701, K252a, UCN-01, rebeccamycin and K252c, with protein kinases by Chaperone interaction assay. STS derivatives causing synthetic lethality (red), no effect (black). Color scale bar indicates log2 fold changes of kinase/Cdc37 interactions between inhibitor and DMSO treatments.

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