Figure 1
From: A lignan from Alnus japonica inhibits glioblastoma tumorspheres by suppression of FOXM1
Expression of FOXM1 and β-catenin mRNAs and proteins in GBM tissues and inhibitory effects of DFS on cell survival. (A,B) Levels of expression of (A) FOXM1 and (B) β-catenin (CTNNB1) mRNAs and comparison with public datasets. 865 GBM tissues and 723 normal brain tissues in the dataset of Oncopression, while 228 GBM tissues and 28 normal brain tissues in the dataset of REMBRANDT. (C) Cell viability and (D) ATP levels measured after 72 h of treatment with the indicated concentrations of DFS. Results are expressed as the means ± SD (n = 5). (E,F) GBM TSs were treated with DFS for 72 h, and (E) cell cycle fractions and (F) apoptotic cell populations analyzed by FACS. (G) Expression of apoptosis-associated proteins measured by western blotting after treatment with DFS for 72 h. Differences among groups were compared by one-way ANOVA with Tukey’s post hoc test for multiple comparisons; *P < 0.05, **P < 0.01, ***P < 0.001.
