Figure 1 | Scientific Reports

Figure 1

From: Automated in vivo compound screening with zebrafish and the discovery and validation of PD 81,723 as a novel angiogenesis inhibitor

Figure 1

Top 5 hit compounds from a screen of the NIHCCLI&II for angiogenesis inhibitors. (A) Representative images of Tg(kdrl:EGFP) zebrafish treated with the top 5 hit compounds. Pixels containing endothelial cells are identified and enhanced with the HTP pixel count readout protocol as previously described 14. Fish are at 4 dpf. Scale bar is 1 mm. (B) Pixel count readout of Tg(kdrl:EGFP) zebrafish treated with various concentrations of the top 5 compounds compared to a negative control. PD 81,723 (4, 8, and 16 µM) and raclopride (1, 4, and 16 µM) yielded significantly (** = P < 0.01 and * = P < 0.05 vs. control) lower pixel counts when compared to the control group. The experiments were repeated in triplicate with an N = 16 for each condition in each experiment. The error bars represent the standard deviation of the means from the three experiments. (C) Survival curves. None of the compounds seemed to have a major impact on mortality. The error bars represent the standard deviation of the survival percentage from the three experiments.

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