Figure 5 | Scientific Reports

Figure 5

From: Automated in vivo compound screening with zebrafish and the discovery and validation of PD 81,723 as a novel angiogenesis inhibitor

Figure 5

Phosphorylated H3 staining in SIVs and ocular area of 4 dpf zebrafish. (A) Representative images of SIVs and the ocular area of 4 dpf zebrafish treated with 0.05% DMSO, 64 µM PD 81,723, and 8 μM I3M at 10–11 hpf. Scale bars are 50 µm. (B) Graphical representations of the results from three different groups for the phosphorylated H3 stains that were repeated in triplicate with an N = 3 for each group. The error bars represent the standard deviation of the means from the three experiments. The number of endothelial nuclei was significantly reduced in both regions of interest in all instances of this experiment (** = P < 0.01 vs. control). A significant reduction in mitotic activity in the tissue surrounding the ocular vasculature (** = P < 0.01 vs. control) and the SIVs (* = P < 0.05 vs. control) in PD 81,723 fish was observed, when compared to the DMSO group.

Back to article page