Figure 7 | Scientific Reports

Figure 7

From: Automated in vivo compound screening with zebrafish and the discovery and validation of PD 81,723 as a novel angiogenesis inhibitor

Figure 7

Annexin V/propidium iodide (PI) flow cytometry apoptosis assay with HUVECs. (A) Representative scatter plots divided into quadrants showing the distribution of HUVECs marked with annexin V, PI, and double stained with annexin V and PI. The apoptotic activity is quantified by the amount of cells in Q2 and Q2-1. The top plots are controls and the bottom plots are for cells treated with PD 81,723. The plots on the left are for cells grown in complete media and the plots on the right are for cells grown in VEGF-A deficient media. (B) Bar graph exhibiting the amount of apoptotic activity for each condition. There was no significant change in the levels of apoptotic events in PD 81,723 treated cells, independent of the addition of VEGF-A to the growth media. This experiment was repeated three times and the error bars represent the standard deviation from three experiments.

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