Figure 6 | Scientific Reports

Figure 6

From: Salvianolic acid B inhibits RAW264.7 cell polarization towards the M1 phenotype by inhibiting NF-κB and Akt/mTOR pathway activation

Figure 6

Sal B inhibits Akt/mTOR pathway activation induced by LPS + IFN-γ. RAW264.7 macrophages were treated with Sal B (100, 150 or 200 μM) for 1 h and then stimulated with LPS (100 ng/mL) + IFN-γ (2.5 ng/mL) for 24 h. A–C p-Akt (A, B) and p-mTOR (A, C) protein expression was analysed by western blotting. RAW264.7 macrophages were treated with Sal B (200 μM) for 1 h and then stimulated with LPS (100 ng/mL) + IFN-γ (2.5 ng/mL) + insulin (1 μg/mL) for 24 h. D-F p-Akt (D,E) and p-mTOR (D,F) protein expression was analysed by western blotting. The values are presented as the means ± SEMs. N = 3, p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***).

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