Figure 1
From: High-fat diet causes mechanical allodynia in the absence of injury or diabetic pathology
A short term HFD regime does not induce the development of obesity nor hyperglycemia. (a) Mice were assigned either a HFD or chow diet at 6 weeks of age for 9 weeks. Weight assessment was performed weekly and blood glucose measurements were performed every weeks starting at week zero. On week 8, mice received a subthreshold dose (0.005 mg/mL) of PGE2 via intraplantar injection and mechanical hypersensitivity was measured afterwards using von Frey. In addition, serum was collected on week 8 and 9 to assess plasma FFA levels. On week 9, tissues were collected and were used either for calcium imaging or immunohistochemistry. (b, c) HFD males (n = 14) did not gain significantly more weight than their chow counterparts (n = 14) whereas HFD females (n = 15) did gain significantly more weight than their chow counterparts (n = 14); both did not reach levels of obesity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (Two-way ANOVA with Sidak’s post hoc comparison). (d, e) No significant developments occurred in their fasting glucose levels between HFD males (n = 7) and chow males (n = 7) nor between HFD females (n = 8) and chow females (n = 8).
