Figure 4
From: High-fat diet causes mechanical allodynia in the absence of injury or diabetic pathology
A short-term HFD regime induces ATF3 upregulation in lumbar DRGs. (a) Ipsilateral lumbar (L3-L5) DRGs from both diet groups were immunostained with DAPI (blue), IB4+ (green), and ATF-3 (purple). (b) There was a diet-dependent increase in ATF3 expression in HFD males (n = 6) and females (n = 6) compared to their chow male (n = 4) and female (n = 3) counterparts in both ipsilateral and contralateral lumbar DRG; however, only the HFD males had a significant ATF3 upregulation compared to their chow males in the ipsilateral DRG. There was no significant difference in ATF3 expression between ipsilateral and contralateral DRG. *p < 0.05 (Three-way ANOVA with Sidak’s post hoc multiple comparison). (c) There was an observed increase in ATF3 expression in IB4+ nociceptors in HFD males and females compared to their chow counterparts, but it did not reach the threshold of significance. Scale bar: 50 μm.
