Table 1 Primary antibodies.

From: High-fat diet causes mechanical allodynia in the absence of injury or diabetic pathology

Antibody

Vendor

Raised in

Concentration

Isolectin GS-IB4, biotin-xx conjugates

Invitrogen (I21414)

N/A

1:500

ATF-3

Abcam (ab207434)

Rabbit

1:500

GFAP

Dako (Z0334)

Rabbit

1:500

NeuN

EMD Millipore Corporation (MAB377)

Mouse IgG1

1:500

CD68

Bio-Rad (MCA1957)

Rat IgG2a

1:500

DAPI

Sigma

N/A

1:5000

  1. All primary antibodies were made in the corresponding blocking/permeabilization buffer. CaCl2 was added alongside isolectin GS-IB4, biotin-xx conjugates. DAPI was diluted in 1 \(\times\) PBS.
Back to article page