Figure 7
LrB inhibits MAPK and NFATc1 signaling pathways. LrB was used to pretreat the BMMs for 1 h followed by 0, 5, 10, 20, 30 and 60 min. Total cellular proteins were extracted using RIPA lysis buffer and cell lysates were analyzed by Western blotting using primary antibodies specific to p-p38, p38, p-JNK and JNK. The ratios of p-p38/p38 and p-JNK/JNK show the inhibitory effect of LrB on MAPK pathway signaling specific to p-p38 and p-JNK (n = 3).
