Figure 4

Sucrose density analysis and TEM analysis of SARS-CoV-2 particles. (a) Sucrose density analysis of capsid assembly in the presence of 1 × PBS, CPC (50 μg/mL) and Triton X-100 (1%). SARS-CoV-2 Wuhan strain was treated with described regents for 10 min, and the treated virions were applied to the density-gradient ultracentrifugation. Each fraction was applied to SDS-PAGE and analyzed by Western blotting with antibodies against S protein and N protein. (b) Electron micrographs of SARS-CoV-2 virions after treatment with reagents. SARS-CoV-2 Wuhan strain was treated with 1 × PBS, CPC (10, 50, 250 μg/mL) and Triton X-100 (1%) for 10 min at room temperature. Each scale bar represents 50 nm.