Figure 3
From: H-ABC tubulinopathy revealed by label-free second harmonic generation microscopy
Cells that emit second harmonic signal are located in hypomyelinated regions of taiep rats. (A, B) SHG images from an acute preparation of the CC of a 10 months control and mutant rat, respectively. (D, E) Confocal images of fluorescently labeled myelin (red), neurofilaments (green), and nuclei (blue) in the CC of WT (D) and TUBB4A mutant rat (E). The membrane-associated signal appears more intense and organized in control rats than in the mutants and the signal from neurofilaments is less intense in control rats. (C) and (F) Fluorescence of neurofilaments (green dots) and myelin (red dots), (4 WT and 4 taiep rats, *p < 0.05, p = 0.03, two-tailed Mann–Whitney test). The direction of laser polarization for SHG microscopy coincides with the scale bar orientation. The average power used for SHG imaging was 13 mW.
