Figure 5
From: H-ABC tubulinopathy revealed by label-free second harmonic generation microscopy
In cerebellar white matter, differences in cellular numbers between taiep and WT are more pronounced. (A–D) More nuclei are observed in the corpus callosum and cerebellar WM of taiep rats than in WT. (E, F) Tubulin staining in the corpus callosum of WT (E) and taiep rats (F); inset in (F) shows a magnification of the region pointed by the arrow. (G, H) Cerebellar WM and granular layer in WT (G) and taiep rats (H), arrows in (H) point at cell bodies heavily stained for tubulin. (I-L) Sections labeled with the mature oligodendrocyte marker CC1 in red, and tubulin in green; corpus callosum of WT (I) and taiep rats (J), cerebellar WM of WT (K) and taiep rats (L). The arrow in (J) points to a cell positive for tubulin and the arrow in (L) points to a cell positive for tubulin and CC1. (M) Quantification of DAPI, tubulin and CC1 positive cells/mm2 in the corpus callosum and in the cerebellar white matter. Significant differences were found between genotypes for DAPI (+) cells (F(1,8) = 9.35, p = 0.00028) and for tubulin (+) cells (F(1,8) = 37.389, p = 0.015), (n = 3 WT and 3 taiep rats, two-way ANOVA followed by Tukey's multiple comparison test was performed. Different letters indicate significant differences between groups).
