Table 5 Success of reintroduction of Pulsatilla patens on Sadowa Góra in Jaworzno—rate of surviving plants in two subsequent seasons (March/April 2021/2022) after the reintroduction.

From: Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Plots for plants transplantation (October 2020)

No. of transplanted plants in 2020 and plant origin

Seasons 2021/2022

No. of plants survivinga

No. of plants at generative stage

No. of flowering shoots (in different clumps)

No. of fruiting shoots (in different clumps)

1. Slightly recessed area located at the top of a small mound formed after calamine mining; area completely exposed

26

Nine from seeds germinated in vitro

Two from seeds coll. in Kolimagi germinated in garden soil

Six from seeds coll. in Łagiewniki germinated in garden soil

Five from cutting rhizomes

Four regenerated in vitro

5/5

4a/5

7a/17

6a/16

2. A periphery of the excavation with SE exposure and a slope of approx. 10°; area completely exposed

15

Four from seeds germinated in vitro

Four from seeds coll. in Łagiewniki germinated in garden soil

Seven regenerated in vitro

10/9

3b/7

6b/27

6b/23

3. A periphery of the small excavation with NW exposure and an inclination of approx. 25°; area partially shaded

8

One from seeds germinated in vitro

One from seeds germinated in peat

Two from seeds coll. in Łagiewniki germinated in garden soil

Four regenerated in vitro

2/0

0/0

0/0

0/0

4. A periphery of the shallow excavation with exposure E and SE and an inclination of approx. 10°; area partially shaded by Scots pine trees growing in the vicinity

35

Seven from seeds germinated in vitro

Eight from seeds coll. in Kolimagi germinated in garden soil

Nighteen from seeds coll. in Łagiewniki germinated in garden soil

One regenerated in vitro

12/8 (4 clumps dug up by visitors)

3b/6

7/11

6/10

5. A periphery of the shallow with Scots pine in the center; SE exposure and a slope of approx. 15°; area partially shaded by Scots pine

22

Five from seeds coll. in Kolimagi germinated in garden soil

Twelve from seeds coll. in Łagiewniki germinated in garden soil

Five regenerated in vitro

7/7

2b/6

3/16

2/11

6. A periphery of the shallow excavation with SE exposure and an inclination of approx. 25°; area completely exposed

26

Two from seeds coll. in Kolimagi germinated in garden soil

Fifteen from seeds coll. in Łagiewniki germinated in garden soil

Nine regenerated in vitro

4/4

1b/4

1/20

1/19

Total

132

40 [30.33]/33 [25.00]

13/28

24/91

21/79

  1. aPlants from cutting rhizomes or from two-year-old plants from seeds collected in Kolimagi (Natura 2000 “Sasanki w Kolimagach”, NE Poland).
  2. bTwo-year-old plants from seeds collected in Kolimagi.