Figure 7
From: Altered sialidase expression in human myeloid cells undergoing apoptosis and differentiation
Changes in HL60 Cell Sialylation During Differentiation. (A–C) HL60 cells were cultured for 1, 3, 5, and 7 days in the presence of DMF or medium alone, lysed, and the lysates, at 50 µg total cellular protein/lane, were resolved by SDS-PAGE, transferred to PVDF, and probed with biotinylated MAL (A), SNA (B), or PNA (C), each followed by incubation with HRP-conjugated streptavidin and ECL reagents. To control for protein loading and transfer, blots were stripped and reprobed for β-actin. IB = immunoblot; IB* = immunoblot after stripping. MW in kDa indicated on left. Arrows on right indicate unidentified bands in which sialylation changes over time. Each lectin blot is representative of 3 independent experiments. Cropped lectin blot images are shown.
