Figure 1 | Scientific Reports

Figure 1

From: Alpinumisoflavone ameliorates choroidal neovascularisation and fibrosis in age-related macular degeneration in in vitro and in vivo models

Figure 1

Inhibitory effects of alpinumisoflavone (AIF) on THP-1 conditioned media (TCM)-induced expression of vascular endothelial growth factor A (VEGFA) in ARPE-19 cells. (a) ARPE-19 cells were treated with 0.1% DMSO in RPMI-1640 complete media or AIF (0, 1, 5, or 10 µM) in TCM. VEGFA expression was measured by dot blot. The data were analysed using one-way ANOVA followed by the Holm-Šídák post-hoc t-test (*p < 0.05 vs. non-TCM group, ##p < 0.01 vs. cells treated with only TCM group) and are expressed as mean ± S.E. (n = 3–6). Uncropped blot images are presented in supplementary information (Supplementary Fig. 2). (b,c) ARPE-19 cells were treated with 0.1% DMSO in RPMI-1640 complete media or AIF (0, 0.1, 1, 5, or 10 µM) in TCM. The fluorescence intensity was measured using the FlexStation 3 Multi-Mode Microplate Reader. Data were analysed using one-way ANOVA followed by the Holm-Šídák post-hoc t-test test (*p < 0.05 vs. non-TCM group, #p < 0.05 vs. cells treated with only TCM group) and are expressed as the mean ± S.E. (n = 6–9).

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