Figure 7

Inhibitory effects of alpinumisoflavone (AIF) on crystallin alpha B (CRYAB) and phosphorylated-CRYAB (p-CRYAB) expression in laser-induced choroidal neovascularisation (CNV) model. Intravitreal injection of 25 mg/mL AIF was administered immediately after laser exposure. The tissue lysates from three animals were pooled as one sample and equal amounts of protein were analysed for determining expression of the indicated proteins. The cleaved form of (a) CRYAB and (b) p-CRYAB reduced by 25 mg/mL AIF treatment in the laser-induced CNV model. Experiments were performed in triplicate and data were analysed using one-way ANOVA followed by the Holm-Šídák post-hoc t-test (**p < 0.01 vs. non-laser exposed group, ^##p < 0.01 vs. only Laser exposed group). The results are expressed as mean ± S.E. (n = 3). Each error bars represent standard deviation from triplicate reading of three pooled samples. Uncropped blot images are presented in supplementary information (Supplementary Fig. 4).