Table 1 Forty-eight-hour in vitro fermentation parameters of three proportions of sericea lespedeza [SL; Lespedeza cuneata (Dum. Cours.) G. Don] hay with bermudagrass[Cynodon dactylon (L.) Pers.] hay diets from Trial 1.

From: Stable isotopes provide evidence that condensed tannins from sericea lespedeza are degraded by ruminal microbes

Item

Treatmentsa

SEM

P value

0SL

50SL

100SL

L

Q

pH

6.63

6.67

6.77

0.011

 < 0.001

 < 0.001

NH3–N, mM

1.18

1.15

1.10

0.031

0.001

0.076

GP, mL/g OM

59.45

49.89

36.14

7.678

 < 0.001

0.071

IVDOM, %

41.14

38.49

33.31

1.956

 < 0.001

0.061

GP/IVDOM, mL/g

1.44

1.29

1.08

0.216

 < 0.001

0.468

Total CH4, mL/g OM

2.83

1.68

1.34

0.708

0.006

0.109

CH4/GP, %

4.76

3.37

3.71

0.041

0.004

0.005

CH4/IVDOM, mL/g

6.88

4.36

4.02

1.75

0.025

0.305

δ13C–CH4, ‰

− 42.61

− 42.94

− 51.33

3.012

0.006

0.917

  1. GP, gas production; IVDOM, in vitro digestible organic matter; NH3–N, ammonia–nitrogen; CH4, methane; SEM, standard error of the mean; L, linear effect of SL inclusion; Q, quadratic effect of SL inclusion.
  2. a0SL: 0% (fresh weight basis) sericea lespedeza hay (100% bermudagrass hay); 50SL: 50% sericea lespedeza hay + 50% bermudagrass hay; 100SL: 100% sericea lespedeza hay (0% bermudagrass hay).
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