Figure 3 | Scientific Reports

Figure 3

From: A systematic exploration reveals the potential of spermidine for hypopigmentation treatment through the stabilization of melanogenesis-associated proteins

Figure 3

SPD treatment improves the stabilities of tyrosinase-related protein (TRP)-1 and TRP-2. (a) Western blots of B16F10 cells treated with 5 μM of SPD for 2 days and subsequent treatment with 10 μM of MG132 for 2 h. Samples were derived from the same experiment and blots were processed in parallel. Original blots are presented in Fig. S1. The data represent the results of three independent experiments. (b) TA99 protein levels examined by confocal microscopy at a magnification of 400× in MNT-1 cells treated with 2 μM of SPD for 10 days. Cell nuclei (blue) were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI). (c) Western blotting of MNT-1 cells with treated with 10 μM of CHX for the indicated time periods and subsequent treatment with 2 μM of SPD. Samples were derived from the same experiment and blots were processed in parallel. Original blots are presented in Fig. S2. The data represent the results of three independent experiments. CTL: control, NS; non-significant, *P < 0.05, **P < 0.01, ***P < 0.005.

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