Figure 7

Multiphoton — Aging differences in the 3D density and organization parameters of elastin and collagen fibers networks. Data within the 50-µm thick dermal sublayer from 3 clinical trials: study I acquired with DermaInspect on ventral forearm (30–40 vs 55–65 year-old; “light” ITA vs “tan” ITA skin color groups); study II acquired with DermaInspect on ventral and dorsal forearms (18–25 vs 70–75 year-old); study III acquired with MPTflex on dorsal forearm and face temple area (18–25 vs 70–75 year-old). (a1–a3) 3D Mean 2PEF elastin fibers density; (b1–b3) 3D Mean SHG collagen fibers density; (c1–c3) 3D Mean SHGto2PEF fibrillar collagen to elastin densities ratio; (d1–d3) 3D Mean SAAID fibrillar collagen and elastin density-based index; (e1–e3) 3D Mean ImbrN fibrillar collagen and elastin normalized imbrication index. The raw data are expressed as box plots with fences, mean and median (—). All ROIs values and their distribution (dots () and histogram) are shown on the right side of the boxplot. Overlapping values are highlighted in black (♦). In study I, the pink and green data points correspond to respectively women and men volunteers. The mean and median values with their errors are given in Table S2. All the statistical p-values and ES—effect sizes parameters are given in Table S3. Statistically significant p-values: *≤ 0.05; **≤ 0.01, ***≤ 0.001. The colored brackets indicate the ES—effect size (dark green—very strong [1.3–Inf], light green—strong [0.8–1.3] and yellow—moderate [0.5–0.8]). Only p-values associated with moderate to very strong ES are shown.