Table 3 Ranking of candidate RGs stability according to tested algorithms for whole A. sativa–P. coronata pathosystem and specific samples subgroups.
Method | < –- Most stable genes Least stable genes –- > | ||||||||
|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
All samples combined | |||||||||
geNorm | ARF/HNR | – | EF1A | UBC | EIF4A | TUA | HSP70 | GAPDH | CYP |
NormFinder | HNR | EIF4A | EF1A | ARF | HSP70 | TUA | UBC | CYP | GAPDH |
BestKeeper | EF1A | HNR | EIF4A | ARF | UBC | TUA | HSP70 | GAPDH | CYP |
ΔCt method | HNR | ARF | EF1A | EIF4A | UBC | TUA | HSP70 | GAPDH | CYP |
A. sativa–P. coronata compatible interaction | |||||||||
geNorm | ARF/HNR | – | EF1A | UBC | EIF4A | HSP70 | TUA | GAPDH | CYP |
NormFinder | ARF | EIF4A | EF1A | HNR | UBC | HSP70 | TUA | GAPDH | CYP |
BestKeeper | EF1A | ARF | EIF4A | HNR | UBC | HSP70 | TUA | GAPDH | CYP |
ΔCt method | ARF | EF1A | HNR | EIF4A | UBC | HSP70 | TUA | GAPDH | CYP |
A. sativa–P. coronata incompatible interaction | |||||||||
geNorm | CYP/EIF4A | – | GAPDH | ARF | HNR | UBC | EF1A | TUA | HSP70 |
NormFinder | ARF | HNR | GAPDH | EIF4A | EF1A | UBC | CYP | TUA | HSP70 |
BestKeeper | EF1A | EIF4A | ARF | HNR | TUA | UBC | GAPDH | CYP | HSP70 |
ΔCt method | ARF | HNR | EIF4A | GAPDH | EF1A | UBC | CYP | TUA | HSP70 |
Pc39 oat line | |||||||||
geNorm | ARF/HNR | – | EF1A | UBC | EIF4A | TUA | HSP70 | CYP | GAPDH |
NormFinder | HNR | ARF | EIF4A | EF1A | UBC | TUA | CYP | HSP70 | GAPDH |
BestKeeper | EF1A | EIF4A | HNR | ARF | UBC | TUA | HSP70 | CYP | GAPDH |
ΔCt method | HNR | ARF | EF1A | EIF4A | UBC | TUA | CYP | HSP70 | GAPDH |
Kasztan cultivar | |||||||||
geNorm | EIF4A/HSP70 | – | HNR | ARF | EF1A | UBC | TUA | GAPDH | CYP |
NormFinder | HNR | EIF4A | HSP70 | ARF | EF1A | TUA | GAPDH | UBC | CYP |
BestKeeper | EF1A | HNR | EIF4A | ARF | HSP70 | UBC | TUA | GAPDH | CYP |
ΔCt method | HNR | EIF4A | HSP70 | ARF | EF1A | TUA | UBC | GAPDH | CYP |
