Figure 7
From: Inflammatory cytokines directly disrupt the bovine intestinal epithelial barrier
Cytokine treatment alters apoptosis. (A–D) Representative confocal images of cytokine-treated bovine intestinal organoids stained for Cleaved Caspase-3 (CCasp3) (Alexa Fluor 594) and DAPI using a ×63 objective lens. Scale bars denote 50 µm. (E) Area of intraluminal CCasp3 normalized to DAPI measured by confocal microscopy. C1, C2, and C3 indicate individual enteroid lines. IFNγ induces a rise in apoptotic cells (p = 0.0325) determined by nested ANOVA Holm-Šídák post hoc analysis.
