Figure 3
From: Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco
Alignment of eIF4E sequences isolated from the selected T0 lines. (a) eIF4E1-S, (b) eIF4E1-T, (c) eIF4E2-S, (d) eIF4E2-T loci. The sgRNA target sequences and the PAM motifs are highlighted in yellow and blue, respectively. Red letters indicate inserted nucleotides. Red dashed lines represent deleted nucleotides. Δ refers to changes in the CRISPR/Cas9 targeted sequence: 0, no change; − deletion; + insertion. “Clones” indicate the number of clones used for sequencing.
