Figure 4
From: Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco
Effect of eIF4E/eIF(iso)4E mutations on PVY accumulation in T0 tobacco plants. (a) PVYO accumulation in the systemic leaves of infected plants was assessed by DAS-ELISA at 2, 3 and 4 weeks post-inoculation (wpi); WT wild type plant, isoE eIF(iso)4E-S and eIF(iso)4E-T double mutant line, eF-7 eIF4E2-S and eIF4E2-T double mutant line, eF-4 eIF4E1-T mutant line, eF-19 eIF4E1-S mutant line, eF-31 eIF4E1-S and eIF4E1-T double mutant line; eF-10 eIF4E1-S, eIF4E1-T, eIF4E2-S and eIF4E2-T quadruple mutant line; negative control: non-inoculated plant; Mean and standard errors of 405 nm absorbance (A405) values were calculated for 5 independent plants per line, *P < 0.05 versus corresponding positive control (WT). (b) PVYO-infected Nicotiana tabacum K326 plants at 10 wpi.
