Figure 5
From: Intracellular hemin is a potent inhibitor of the voltage-gated potassium channel Kv10.1
Hemin binding to Kv10.1 C-linker peptides confirmed with EPR spectroscopy. EPR measurements of 22-mer C-linker peptides encompassing the heme-binding residues C541, H543, and H552 of the indicated peptides (125 µM) and hemin (100 µM) at 9 K. Arrows mark the g factor 2.23, representing rhombic signals typical for axial ligation of the low-spin hemin iron. Abbreviations used: (wt) CHH, (C541A:H543A:H552A) AAA, (C541A) AHH, (H543A) CAH, (H552A) CHA, and (H543A:H552A) CAA. (top, left) Sequences for the wild-type (CHH) and mutant AAA peptide.
