Figure 7
Representative confocal images of double cytokeratin and vimentin (A) β-catenin (B) or E-cadherin (C) immunofluorescence corresponding to 2D endometrial epithelial monolayers isolated from Cre:ER+/−; SMAD2f/f; SMAD3f/f. Cells were plated and treated with tamoxifen (TAM) to induce Cre:ER-mediated deletion of SMAD2 and SMAD3 alleles or left untreated (NO TAM) and then stimulated for 72 h with 20 ng/ml of TGF-β. Images were captured after 3 days of tamoxifen-induced deletion. Cells were counterstained with Hoechst to visualize nuclei. Scale bars: 25 µm.
