Figure 3
From: Fat3 regulates neural progenitor cells by promoting Yap activity during spinal cord development
Fat3 is crucial for proper ventral progenitor formation in developing mouse spinal cord. (A) The expression of proliferation markers (pH3, BrdU, Sox2) and neuronal markers (NeuN and TuJ) in transverse sections at E11.5 in Fat3f/f;N-Cre embryos (n = 3) and their littermate controls (n = 4) by immunostaining analyses with indicated antibodies for mouse embryos (cervical level). IHC with anti-Fat3 antibody confirms the absence of Fat3 expression in Fat3f/f;N-Cre embryos. Quantification was performed with 5–10 images from multiple embryos as indicated and at least three sections from each embryo. Values are means ± sem. *p < 0.05, **p < 0.01 (Student’s t-test); ns not significant. Dotted outlines indicate the border of the spinal cord. Scale bars: 100 μm.
